Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: concepts and applications

Covering 2011 to 2022 Low titers of natural products in laboratory culture or fermentation conditions have been one of the challenging issues in natural products research. Many natural product biosynthetic gene clusters (BGCs) are also transcriptionally silent in laboratory culture conditions, makin...

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Bibliographic Details
Published in:Natural product reports 2024-04, Vol.41 (4), p.672-699
Main Authors: Ji, Chang-Hun, Je, Hyun-Woo, Kim, Hiyoung, Kang, Hahk-Soo
Format: Article
Language:English
Subjects:
Actinobacteria - genetics
Actinobacteria - metabolism
Actinomycetes
Biological Products - metabolism
Biosynthetic Pathways - genetics
Fermentation
Gene clusters
Genetic Engineering - methods
Laboratory culture
Metabolites
Molecular Structure
Multigene Family
Natural products
Promoter Regions, Genetic
Promoters
Transcription activation
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Summary:Covering 2011 to 2022 Low titers of natural products in laboratory culture or fermentation conditions have been one of the challenging issues in natural products research. Many natural product biosynthetic gene clusters (BGCs) are also transcriptionally silent in laboratory culture conditions, making it challenging to characterize the structures and activities of their metabolites. Promoter engineering offers a potential solution to this problem by providing tools for transcriptional activation or optimization of biosynthetic genes. In this review, we summarize the 10 years of progress in promoter engineering approaches in natural products research focusing on the most metabolically talented group of bacteria actinomycetes. Promoter engineering has been used to improve production titers of natural products or to activate transcriptionally silent BGCs. This review summarizes 10 years of progress in promoter engineering approaches in natural products research.
ISSN:0265-0568
1460-4752
1460-4752
DOI:10.1039/d3np00049d