First report of a bla NDM-5 -carrying Escherichia coli sequence type 12 isolated from a dog with pyometra in Japan

• Published in: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2024-09, Vol.30 (9), p.938
• Main Authors: Harada, Kazuki, Miyamoto, Tadashi, Sugiyama, Michiyo, Asai, Tetsuo
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; beta-Lactamases - genetics; Dog Diseases - drug therapy; Dog Diseases - microbiology; Dogs; Drug Resistance, Multiple, Bacterial - genetics; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli Infections - drug therapy; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Female; Japan; Microbial Sensitivity Tests; Plasmids - genetics; Pyometra - drug therapy; Pyometra - microbiology; Pyometra - veterinary
• ISSN: 1437-7780
• DOI: 10.1016/j.jiac.2024.02.013

Carbapenemase-producing Enterobacterales (CPE) are a serious concern in human clinical settings. Companion animal-origin CPE have been only rarely identified in several countries, but they have not yet been identified in Japan. In this study, we present the first case of a canine infected with CPE in Japan. The patient was hospitalized due to pyometra. The pus discharged from the patient's uterus was subjected to bacteriological analysis. As a result, E. coli was identified in the pus and exhibited resistance to piperacillin, amoxicillin-clavulanic acid, cefazolin, ceftazidime, cefepime, meropenem, amikacin, and sulfamethoxazole-trimethoprim and susceptibility to aztreonam, minocycline, and levofloxacin. Results of the sodium mercaptoacetic acid double-disk synergy test showed that the E. coli isolate was positive for metallo-β-lactamases. Next-generation sequencing identified the bla gene, which was located in the IncFII-type plasmid together with bla , rmtB, aadA2, bleMBL, sul1, qacE, and dfrA12. The case was treated successfully with doxycycline and orbifloxacin. Our finding emphasizes that close attention should be paid to the significance of CPE harboring multidrug-resistance plasmid in companion animals, based on the perspective of One Health approach in Japan as well as in other countries.