Droplet microfluidic system for high throughput and passive selection of bacteria producing biosurfactants

• Published in: Lab on a chip 2024-03, Vol.24 (7), p.1947-1956
• Main Authors: Staskiewicz, Klaudia, Dabrowska-Zawada, Maria, Kozon, Lukasz, Olszewska, Zofia, Drewniak, Lukasz, Kaminski, Tomasz S
• Format: Article
• Language: English
• Subjects: Bacteria; Bioremediation; Biotechnology; Droplets; Enrichment; Microfluidics; Microfluidics - methods; Microorganisms; Production methods; Surface tension; Surface-Active Agents
• ISSN: 1473-0197; 1473-0189; 1473-0189
• DOI: 10.1039/d3lc00656e

Traditional methods for the enrichment of microorganisms rely on growth in a selective liquid medium or on an agar plate, followed by tedious characterization. Droplet microfluidic techniques have been recently used to cultivate microorganisms and preserve enriched bacterial taxonomic diversity. However, new methods are needed to select droplets comprising not only growing microorganisms but also those exhibiting specific properties, such as the production of value-added compounds. We describe here a droplet microfluidic screening technique for the functional selection of biosurfactant-producing microorganisms, which are of great interest in the bioremediation and biotechnology industries. Single bacterial cells are first encapsulated into picoliter droplets for clonal cultivation and then passively sorted at high throughput based on changes in interfacial tension in individual droplets. Our method expands droplet-based microbial enrichment with a novel approach that reduces the time and resources needed for the selection of surfactant-producing bacteria. Novel microfluidic technique for functional selection of biosurfactant-producing microorganisms. Single bacterial cells are encapsulated into picoliter droplets for clonal cultivation and passively sorted at high throughput by interfacial tension.