Microbial-transcriptome integrative analysis of heat stress effects on amino acid metabolism and lipid peroxidation in poultry jejunum

Despite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host-microbiome interactions using a pr...

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Bibliographic Details
Published in:Animal biotechnology 2024-11, Vol.35 (1), p.2331179-2331179
Main Authors: Seo, Young-Jun, Lim, Chiwoong, Lim, Byeonghwi, Kim, Jun-Mo
Format: Article
Language:English
Subjects:
Acyl-CoA dehydrogenase
Acyl-CoA oxidase
Amino acids
Biodegradation
Biomarkers
broiler
Cell cycle
Enrichment
Environmental degradation
Gene families
Gene set enrichment analysis
Heat stress
Heat tolerance
Impact analysis
Jejunum
Lipid metabolism
Lipid peroxidation
Lipids
Livestock
Metabolism
Metabolites
microbiome
Microbiomes
Microbiota
Microorganisms
multi-omics integration
Peroxidation
Poultry
Protein metabolism
Protein turnover
RNA-seq
Statistical analysis
Temperature effects
Transcriptomes
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Summary:Despite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host-microbiome interactions using a predicted metabolome data-based approach to understand the impact of heat stress on poultry. After the analysis, the host functional enrichment analysis revealed that pathways related to lipid and protein metabolism were elevated under heat stress conditions. In contrast, pathways related to the cell cycle were suppressed under normal environmental temperatures. In line with the transcriptome analysis, the microbial analysis results indicate that taxonomic changes affect lipid degradation. Heat stress engendered statistically significant difference in the abundance of 11 microorganisms, including Bacteroides and Peptostreptococcacea. Together, integrative approach analysis suggests that microbiota-induced metabolites affect host fatty acid peroxidation metabolism, which is correlated with the gene families of Acyl-CoA dehydrogenase long chain (ACADL), Acyl-CoA Oxidase (ACOX) and Acetyl-CoA Acyltransferase (ACAA). This integrated approach provides novel insights into heat stress problems and identifies potential biomarkers associated with heat stress.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2024.2331179