CO 2 supply is a powerful tool to control homoacetogenesis, chain elongation and solventogenesis in ethanol and carboxylate fed reactor microbiomes

• Published in: Frontiers in bioengineering and biotechnology 2024, Vol.12, p.1329288
• Main Authors: de Leeuw, Kasper D, van Willigen, Marius J W, Vrauwdeunt, Ton, Strik, David P P T B
• Format: Article
• Language: English
• ISSN: 2296-4185; 2296-4185
• DOI: 10.3389/fbioe.2024.1329288

Anaerobic fermentation technology enables the production of medium chain carboxylates and alcohols through microbial chain elongation. This involves steering reactor microbiomes to yield desired products, with CO supply playing a crucial role in controlling ethanol-based chain elongation and facilitating various bioprocesses simultaneously. In the absence of CO supply (Phase I), chain elongation predominantly led to n-caproate with a high selectivity of 96 Cmol%, albeit leaving approximately 80% of ethanol unconverted. During this phase, and -related species dominated the reactors. In Phase II, with low CO input (2.0 NmL L  min ), formation of n-butyrate, butanol, and hexanol was stimulated. Increasing CO doses in Phase III (6 NmL L  min ) led to CO utilization via homoacetogenesis, coinciding with the enrichment of , a bacterium that can use CO as an electron acceptor. Lowering CO dose to 0.5 NmL L  min led to a shift in microbiome composition, diminishing the dominance of while increasing abundance. Additionally, other , , and -related species became prevalent. This decrease in CO load from 6 to 0.5 NmL L  min minimized excessive ethanol oxidation from 30%-50% to 0%-3%, restoring a microbiome favoring net n-butyrate consumption and n-caproate production. The decreased ethanol oxidation coincided with the resurgence of hydrogen formation at partial pressures above 1%. High concentrations of butyrate, caproate, and ethanol in the reactor, along with low acetate concentration, promoted the formation of butanol and hexanol. It is evident that CO supply is indispensable for controlling chain elongation in an open culture and it can be harnessed to stimulate higher alcohol formation or induce CO utilization as an electron acceptor.