Differential extracellular vesicle concentration and their biomarker expression of integrin α v /β 5 , EpCAM, and glypican-1 in pancreatic cancer models

Tumor-derived extracellular vesicles (EVs) show great potential as biomarkers for several diseases, including pancreatic cancer, due to their roles in cancer development and progression. However, the challenge of utilizing EVs as biomarkers lies in their inherent heterogeneity in terms of size and c...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2024-06, Vol.14 (1), p.14273
Main Authors: Jacobson, Reed, Ha, Sangdeuk, Tani, Sakurako, Ghosh, Shrinwanti, Jarajapu, Yagna P R, Brand, Randall E, Kim, Jiha, Choi, Yongki
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor - metabolism
Cell Line, Tumor
Epithelial Cell Adhesion Molecule - metabolism
Extracellular Vesicles - metabolism
Glypicans - metabolism
Humans
Integrin alphaV - metabolism
Mice
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tumor-derived extracellular vesicles (EVs) show great potential as biomarkers for several diseases, including pancreatic cancer, due to their roles in cancer development and progression. However, the challenge of utilizing EVs as biomarkers lies in their inherent heterogeneity in terms of size and concentration, making accurate quantification difficult, which is highly dependent on the isolation and quantification methods used. In our study, we compared three EV isolation techniques and two EV quantification methods. We observed variations in EV concentration, with approximately 1.5-fold differences depending on the quantification method used. Interestingly, all EV isolation techniques consistently yielded similar EV quantities, overall size distribution, and modal sizes. In contrast, we found a notable increase in total EV amounts in samples from pancreatic cancer cell lines, mouse models, and patient plasma, compared to non-cancerous conditions. Moreover, individual tumor-derived EVs exhibited at least a 3-fold increase in several EV biomarkers. Our data, obtained from EVs isolated using various techniques and quantified through different methods, as well as originating from various pancreatic cancer models, suggests that EV profiling holds promise for the identification of unique and cancer-specific biomarkers in pancreatic cancer.
ISSN:2045-2322