Expanding the range of graphene energy transfer with multilayer graphene

• Published in: Nanoscale 2024-07, Vol.16 (28), p.13464-1347
• Main Authors: Gronkiewicz, Karolina, Richter, Lars, Knechtel, Fabian, Pyrcz, Patryk, Leidinger, Paul, Günther, Sebastian, Ploetz, Evelyn, Tinnefeld, Philip, Kami ska, Izabela
• Format: Article
• Language: English
• Subjects: Bilayers; Biophysics; Dyes; Emitters; Energy transfer; Graphene; Microscopy; Multilayers; Nanostructure; Position measurement; Substrates
• ISSN: 2040-3364; 2040-3372; 2040-3372
• DOI: 10.1039/d4nr01723d

The interaction between single emitters and graphene in the context of energy transfer has attracted significant attention due to its potential applications in fields such as biophysics and super-resolution microscopy. In this study, we investigate the influence of the number of graphene layers on graphene energy transfer (GET) by placing single dye molecules at defined distances from monolayer, bilayer, and trilayer graphene substrates. We employ DNA origami nanostructures as chemical adapters to position the dye molecules precisely. Fluorescence lifetime measurements and analysis reveal an additive effect of graphene layers on the energy transfer rate extending the working range of GET up to distances of approximately 50-60 nm. Moreover, we show that switching a DNA pointer strand between two positions on a DNA origami nanostructure at a height of >28 nm above graphene is substantially better visualized with multilayer graphene substrates suggesting enhanced capabilities for applications such as biosensing and super-resolution microscopy for larger systems and distances. This study provides insights into the influence of graphene layers on energy transfer dynamics and offers new possibilities for exploiting graphene's unique properties in various nanotechnological applications. Each additional graphene layer leads to stronger fluorescence quenching, indicating enhanced GET efficiency and a potential extension of the dynamic working range of GET.