m 1 A demethylase Alkbh3 regulates neurogenesis through m 1 A demethylation of Mmp15 mRNA

N -Methyladenosine (m A) is an abundant modification of transcripts regulating mRNA structure and translation efficiency. However, the characteristics and biological functions of mRNA m A modification in adult hippocampal neurogenesis remain enigmatic. We found that m A demethylase Alkbh3 was dramat...

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Published in:Cell & bioscience 2024-07, Vol.14 (1), p.92
Main Authors: Wang, Huan, Xie, Linjie, Guo, Haomin, Li, Lishi, Chen, Shuwei, Fan, Ye, Tian, Jingyuan, Xu, Liping, Kong, Xuejian, Xuan, Aiguo
Format: Article
Language:English
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Summary:N -Methyladenosine (m A) is an abundant modification of transcripts regulating mRNA structure and translation efficiency. However, the characteristics and biological functions of mRNA m A modification in adult hippocampal neurogenesis remain enigmatic. We found that m A demethylase Alkbh3 was dramatically enriched in neurons and neuronal genesis. Functionally, depletion of Alkbh3 in neural stem cells (NSCs) significantly decreased m A modification, neuronal differentiation and proliferation coupling with increasing gliogenesis, whereas overexpressing Alkbh3 facilitated neuronal differentiation and proliferation. Mechanistically, the m A demethylation of Mmp15 mRNA by Alkbh3 improved its RNA stability and translational efficacy, which promoted neurogenesis. Therapeutically, the silencing of Alkbh3 reduced hippocampal neurogenesis and impaired spatial memory in the adult mice. We reveal a novel function of m A demethylation on Mmp15 mRNA in Alkbh3-mediated neurogenesis, which shed light on advancing Alkbh3 regulation of neurogenesis as a novel neurotherapeutic strategy.
ISSN:2045-3701
2045-3701
DOI:10.1186/s13578-024-01275-9