Transglutaminase-mediated proximity labeling of a specific Lys residue in a native IgG antibody

The fusion protein of an engineered zymogen of microbial transglutaminase (EzMTG) with a protein G variant, EzMTG-pG, enabled the proximity-based, tag-free labeling of Lys65 in the heavy chain of a native IgG antibody (trastuzumab) with a Gln-donor peptidyl substrate functionalized with a fluorescen...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2024-08, Vol.6 (65), p.8545-8548
Main Authors: Nishioka, Riko, Iida, Ryuya, Minamihata, Kosuke, Sato, Ryo, Kimura, Michio, Kamiya, Noriho
Format: Article
Language:English
Subjects:
Fluorescence
Fluorescent Dyes - chemistry
Humans
IgG antibody
Immunoglobulin G - chemistry
Immunoglobulin G - metabolism
Labeling
Lysine - chemistry
Microorganisms
Proteins
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - metabolism
Substrates
Transglutaminases - chemistry
Transglutaminases - metabolism
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Summary:The fusion protein of an engineered zymogen of microbial transglutaminase (EzMTG) with a protein G variant, EzMTG-pG, enabled the proximity-based, tag-free labeling of Lys65 in the heavy chain of a native IgG antibody (trastuzumab) with a Gln-donor peptidyl substrate functionalized with a fluorescent molecule. The fusion protein of an engineered microbial transglutaminase and protein G variant enabled site-specific and tag-free labeling of Lys65 of a native IgG with a Gln-donor peptidyl substrate.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d4cc01728e