Hypermutation at the Immunoglobulin Heavy Chain Locus in a Pre-B-Cell Line

Most cells in the well-known pre-B-lymphocyte line 18-81 have correctly assembled genes for both alleles at the immunoglobulin heavy chain locus. Only one allele is ``active''; the other, ``silent'' allele contains an amber termination codon. The rate of reversion of this amber c...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-01, Vol.82 (2), p.479-482
Main Authors: Wabl, Matthias, Burrows, Peter D., Von Gabain, Alex, Steinberg, Charles
Format: Article
Language:English
Subjects:
Alleles
Animals
B lymphocytes
B-Lymphocytes - cytology
Biological and medical sciences
Cell Compartmentation
Cell lines
Cellular immunity
Codon - analysis
Codons
Feeder cells
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression Regulation
Genetic loci
Genetic mutation
Genetics of the immune response
Hybridomas
Immunobiology
Immunoglobulin Heavy Chains - genetics
Mathematics
Mice
Mice, Inbred BALB C
Multiple Myeloma - genetics
Mutation
Plasma cells
Stem Cells - immunology
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Summary:Most cells in the well-known pre-B-lymphocyte line 18-81 have correctly assembled genes for both alleles at the immunoglobulin heavy chain locus. Only one allele is ``active''; the other, ``silent'' allele contains an amber termination codon. The rate of reversion of this amber codon was determined to be 0.3-1 × 10-4per cell generation. This high rate is termed hypermutation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.2.479