Enzyme Assays for the Identification of Gastric Fluid

Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories. These samples are usually vomitus, or stomach contents originating from wounds that perforate the stomach....

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Bibliographic Details
Published in:Journal of forensic sciences 1985-01, Vol.30 (1), p.97-102
Main Authors: Lee, HC, Gaensslen, RE, Galvin, C, Pagliaro, EM
Format: Article
Language:English
Subjects:
Body Fluids - analysis
Chymosin - analysis
Clinical Enzyme Tests
Forensic chemistry
Forensic Medicine
Forensic pathology
Forensic sciences
Gastric Juice - enzymology
Humans
Pepsin A - analysis
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Summary:Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories. These samples are usually vomitus, or stomach contents originating from wounds that perforate the stomach. They may be encountered at scenes or on articles submitted for examination, in fresh form or as dried stains. The pepsin activity assay is based on proteolytic activity with bovine albumin as substrate and the rennin-like activity assay is based on the coagulation of milk protein.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS10969J