PM 2.5 Induces Pyroptosis via Activation of the ROS/NF-κB Signaling Pathway in Bronchial Epithelial Cells

• Published in: Medicina (Kaunas, Lithuania) Lithuania), 2024-09, Vol.60 (9)
• Main Authors: Kang, Ji-Young, Choi, Hyunsu, Oh, Jeong-Min, Kim, Minsu, Lee, Dong-Chang
• Format: Article
• Language: English
• Subjects: Bronchi - drug effects; Bronchi - metabolism; Cell Line; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Humans; Interleukin-18 - metabolism; Interleukin-1beta - metabolism; NF-kappa B - metabolism; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Oxidative Stress - drug effects; Particulate Matter - adverse effects; Pyroptosis - drug effects; Pyroptosis - physiology; Reactive Oxygen Species - metabolism; Signal Transduction - drug effects
• ISSN: 1648-9144
• DOI: 10.3390/medicina60091434

: Fine particulate matter, PM , is becoming a major threat to human health, particularly in terms of respiratory diseases. Pyroptosis is a recently discovered and distinct form of cell death, characterized by pore formation in the cell membrane and secretions of proinflammatory cytokines. There has been little research on the effect of PM on pyroptosis, especially in airway epithelium. We investigated whether PM -related oxidative stress induces pyroptosis in bronchial epithelial cells and defined the underlying mechanisms. : After exposure of a BEAS-2B cell line to PM concentration of 20 µg/mL, reactive oxygen species (ROS) levels, parameters related to pyroptosis, and NF-κB signaling were measured by Western blotting, immunofluorescence, and ELISA (Enzyme-linked immunosorbent assay). : PM induced pyroptotic cell death, accompanied by LDH (Lactate dehydrogenase) release and increased uptake of propidium iodide in a dose-dependent manner. PM activated the NLRP3-casp1-gasdermin D pathway, with resulting secretions of the proinflammatory cytokines IL-1β and IL-18. The pyroptosis activated by PM was alleviated significantly by NLRP3 inhibitor. In PM -exposed BEAS-2B cells, levels of intracellular ROS and NF-κB p65 increased. ROS scavenger inhibited the expression of the NLRP3 inflammasome, and the NF-κB inhibitor attenuated pyroptotic cell death triggered by PM exposure, indicating that the ROS/NF-κB pathway is involved in PM -induced pyroptosis. : These findings show that PM exposure can cause cell injury by NLRP3-inflammasome-mediated pyroptosis by upregulating the ROS/NF-κB pathway in airway epithelium.