Cytokine-armed vaccinia virus promotes cytotoxicity toward pancreatic carcinoma cells via activation of human intermediary CD56 dim CD16 dim natural killer cells

• Published in: International journal of cancer 2025-02, Vol.156 (3), p.638
• Main Authors: Wang, Ruonan, Hu, Mengwen, Lozzi, Isis, Jin, Cao Zhong Jing, Ma, Dou, Splith, Katrin, Mengwasser, Jörg, Wolf, Vincent, Feldbrügge, Linda, Tang, Peter, Timmermann, Lea, Hillebrandt, Karl Herbert, Kirchner, Marieluise, Mertins, Philipp, Hilfenhaus, Georg, Neumann, Christopher Claudius Maximilian, Kammertoens, Thomas, Pratschke, Johann, Malinka, Thomas, Sauer, Igor Maximillian, Noessner, Elfriede, Guo, Zong Sheng, Felsenstein, Matthäus
• Format: Article
• Language: English
• Subjects: Carcinoma, Pancreatic Ductal - immunology; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - pathology; Carcinoma, Pancreatic Ductal - virology; CD56 Antigen - metabolism; Cell Line, Tumor; Coculture Techniques; Cytotoxicity, Immunologic; Humans; Interleukin-15 - metabolism; Interleukin-2 - metabolism; Killer Cells, Natural - immunology; Killer Cells, Natural - metabolism; Oncolytic Virotherapy - methods; Oncolytic Viruses - immunology; Pancreatic Neoplasms - immunology; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - virology; Tumor Microenvironment - immunology; Vaccinia virus - immunology
• ISSN: 1097-0215

Pancreatic ductal adenocarcinoma (PDAC) remains a particularly aggressive disease with few effective treatments. The PDAC tumor immune microenvironment (TIME) is known to be immune suppressive. Oncolytic viruses can increase tumor immunogenicity via immunogenic cell death (ICD). We focused on tumor-selective (vvDD) and cytokine-armed Western-reserve vaccinia viruses (vvDD-IL2 and vvDD-IL15) and infected carcinoma cell lines as well as patient-derived primary PDAC cells. In co-culture experiments, we investigated the cytotoxic response and the activation of human natural killer (NK). Infection and virus replication were assessed by measuring virus encoded YFP. We then analyzed intracellular signaling processes and oncolysis via in-depth proteomic analysis, immunoblotting and TUNEL assay. Following the co-culture of mock or virus infected carcinoma cell lines with allogenic PBMCs or NK cell lines, CD56 NK cells were analyzed with respect to their activation, cytotoxicity and effector function. Both, dose- and time-dependent release of danger signals following infection were measured. Viruses effectively entered PDAC cells, emitted YFP signals and resulted in concomitant oncolysis. The proteome showed reprogramming of normally active core signaling pathways in PDAC (e.g., MAPK-ERK signaling). Danger-associated molecular patterns were released upon infection and stimulated co-cultured NK cells for enhanced effector cytotoxicity. NK cell subtyping revealed enhanced numbers and activation of a rare CD56 CD16 population. Tumor cell killing was primarily triggered via Fas ligands rather than granule release, resulting in marked apoptosis. Overall, the cytokine-armed vaccinia viruses induced NK cell activation and enhanced cytotoxicity toward human PDAC cells in vitro. We could show that cytokine-armed virus targets the carcinoma cells and thus has great potential to modulate the TIME in PDAC.