In vivo 31P-nuclear magnetic resonance study of the response of a murine mammary tumor to different doses of γ-radiation

The response of the s.c. implanted murine mammary carcinoma NU-82 to 10 and 20 Gy of gamma-radiation was followed by in vivo 31P-nuclear magnetic resonance spectroscopy on 48 mice during a period of 2 days. During the first 8 h after a dose of 10 Gy, the ratio of ATP/inorganic phosphate increases to...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1986-03, Vol.46 (3), p.1427-1432
Main Authors: SIJENS, P. E, BOVEE, W. M. M. J, SEIJKENS, D, LOS, G, RUTGERS, D. H
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Aerobiosis
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Cell Cycle
Cell Survival
DNA Repair
Dose-Response Relationship, Radiation
Energy Metabolism
Experimental genital and mammary tumors
Gamma Rays
Glycolysis
Magnetic Resonance Spectroscopy
Mammary Neoplasms, Experimental - metabolism
Mammary Neoplasms, Experimental - radiotherapy
Medical sciences
Mice
Nucleotides - metabolism
Phosphates - metabolism
Phosphocreatine - metabolism
Time Factors
Tumors
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Summary:The response of the s.c. implanted murine mammary carcinoma NU-82 to 10 and 20 Gy of gamma-radiation was followed by in vivo 31P-nuclear magnetic resonance spectroscopy on 48 mice during a period of 2 days. During the first 8 h after a dose of 10 Gy, the ratio of ATP/inorganic phosphate increases to a value of 120 +/- 15% (SE) of the control value. This rise is followed by a decrease of the ratio to 74 +/- 12% after 47 h. However treatment with 20 Gy causes the ratio of ATP/Pi to decrease gradually to a level of 45 +/- 7% after 47 h. Histological investigations demonstrated that radiation-induced necrosis largely contributes to this phenomenon. During the time of observation irradiated tumors displayed no changes in size and intracellular pH. After treatment with 10 Gy as well as with 20 Gy, the phosphodiester resonance decreased in intensity. By 31P-nuclear magnetic resonance spectroscopy on extracts of the NU-82 tumor, two phosphodiesters were identified, glycerophosphocholine and glycerophosphoethanolamine.
ISSN:0008-5472
1538-7445