Dopamine-conjugated extracellular vesicles induce autophagy in Parkinson's disease

The application of extracellular vesicles (EVs) as vehicles for anti-Parkinson's agents represents a significant advance, yet their clinical translation is hampered by challenges in efficient brain delivery and complex blood-brain barrier (BBB) targeting strategies. In this study, we engineered...

Full description

Saved in:
Bibliographic Details
Published in:Journal of extracellular vesicles 2024-12, Vol.13 (12), p.e70018
Main Authors: Sul, Jae Hoon, Shin, Sol, Kim, Hark Kyun, Han, Jihoon, Kim, Junsik, Son, Soyong, Lee, Jungmi, Baek, Seung Hyun, Cho, Yoonsuk, Lee, Jeongmi, Park, Jinsu, Ahn, Donghoon, Park, Sunyoung, Palomera, Leon F, Lim, Jeein, Kim, Jongho, Kim, Chanhee, Han, Seungsu, Chung, Ka Young, Lee, Sangho, Kam, Tae-In, Lee, Yunjong, Kim, Jeongyun, Park, Jae Hyung, Jo, Dong-Gyu
Format: Article
Language:English
Subjects:
Animals
Autophagy
Disease Models, Animal
Dopamine - metabolism
Dopaminergic Neurons - metabolism
Extracellular Vesicles - metabolism
Humans
Male
Mice
Mice, Inbred C57BL
Oxidopamine - pharmacology
Parkinson Disease - metabolism
Parkinson Disease - therapy
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The application of extracellular vesicles (EVs) as vehicles for anti-Parkinson's agents represents a significant advance, yet their clinical translation is hampered by challenges in efficient brain delivery and complex blood-brain barrier (BBB) targeting strategies. In this study, we engineered dopamine onto the surface of adipose-derived stem cell EVs (Dopa-EVs) utilizing a facile, two-step cross-linking approach. This engineering enhanced neuronal uptake of the EVs in primary neurons and neuroblastoma cells, a process shown to be competitively inhibited by dopamine pretreatment and dopamine receptor antibodies. Notably, Dopa-EVs demonstrated increased brain accumulation in mouse Parkinson's disease (PD) models. Therapeutically, Dopa-EVs administration led to the rescue of dopaminergic neuronal loss and amelioration of behavioural deficits in both 6-hydroxydopamine (6-OHDA) and α-Syn PFF-induced PD models. Furthermore, we observed that Dopa-EVs stimulated autophagy evidenced by the upregulation of Beclin-1 and LC3-II. These findings collectively indicate that surface modification of EVs with dopamine presents a potent strategy for targeting dopaminergic neurons in the brain. The remarkable therapeutic potential of Dopa-EVs, demonstrated in PD models, positions them as a highly promising candidate for PD treatment, offering a significant advance over current therapeutic modalities.
ISSN:2001-3078