Development of an LC-MS/MS method for the simultaneous quantification of 11 perfluoroalkyl compounds in mouse plasma for toxicokinetic applications

• Published in: Journal of pharmaceutical and biomedical analysis 2024-11, Vol.255, p.116596, Article 116596
• Main Authors: Argoul, Chloé ML, Dauwe, Yannick, Lakhal, Laïla, Toutain, Pierre-Louis, Picard-Hagen, Nicole, Gayrard, Véronique, Lacroix, Marlène Z.
• Format: Article
• Language: English
• Subjects: Chemical Sciences; Life Sciences; Liquid chromatography; Mass spectrometry; Perfluoroalkyl substances; Toxicokinetic
• ISSN: 0731-7085; 1873-264X; 1873-264X
• DOI: 10.1016/j.jpba.2024.116596

Following regulatory pressure, the manufacture of long-chain per- and polyfluoroalkyl substances (PFAS) has been phased out, and alternatives such as short-chain homologs and ether-PFAS have replaced the bioaccumulative long-chain PFAS. However, data are lacking regarding the toxicokinetic (TK) properties of certain PFAS, particularly emergent substitutes for long-chain compounds. Additionally, the existing analytical methods used for TK studies measure a single compound or only a few simultaneously. For this reason, an LC-MS/MS method was developed for the simultaneous quantification in mouse plasma of 11 PFAS representative of some of the most important categories of these compounds, for application in TK studies. The method was successfully validated in the range of 0.5–1000 ng/mL, in accordance with the European Medicines Agency guidelines, and applied to a 24-h pilot TK study conducted in mice. All compounds were monitored over 24 hours in the pilot study. The present method is therefore suitable for the simultaneous quantification of PFAS in plasma samples and can be applied for future TK studies. [Display omitted] •Simultaneous quantification of 11 PFASs for toxicokinetic applications.•Better control of system contamination thanks to inversion of analytical and delay columns.•First exposure results for an emerging PFAS (PFO2OA).