19 F-PCS measurements on proteins in live mammalian cells

Pseudocontact shift (PCS) contains rich structural information of proteins in structural and chemical biology. F-PCS is determined in live mammalian cells dual labelling of the target protein with a paramagnetic tag and a F-tag, which is achieved by varied reactivity of solvent exposed cysteines in...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2024-12
Main Authors: Guo, Shu-Li, Pan, Bin-Bin, Li, Xia-Yan, Xiao, Yu-Hao, Su, Xun-Cheng
Format: Article
Language:English
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Summary:Pseudocontact shift (PCS) contains rich structural information of proteins in structural and chemical biology. F-PCS is determined in live mammalian cells dual labelling of the target protein with a paramagnetic tag and a F-tag, which is achieved by varied reactivity of solvent exposed cysteines in selection of different types of tags. About 0.1 ppm F-PCS can be observed over 20 Å from the paramagnetic center in the live cells.
ISSN:1364-548X