Whole genome analysis revealed the role of bla OXA-23 and bla OXA-66 genes in carbapenem resistance of Acinetobacter baumannii strains

is a multidrug-resistant bacterium that has emerged as a significant nosocomial pathogen globally and renowned for its ability to acquire antimicrobial resistance (AMR) genes. However, understanding of its resistance mechanisms to certain drug classes remains limited. This study focused on four bact...

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Published in:Pathogens and global health 2024-12, p.1
Main Authors: Mat Ghani, Nurizati, Hong, Kar-Wai, Liew, Yvonne Jing Mei, Lau, Yin Yin, Yong, Hoi-Sen, Tee, Kok Keng, Chan, Kok-Gan, Chua, Kah-Ooi
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Language:English
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Summary:is a multidrug-resistant bacterium that has emerged as a significant nosocomial pathogen globally and renowned for its ability to acquire antimicrobial resistance (AMR) genes. However, understanding of its resistance mechanisms to certain drug classes remains limited. This study focused on four bacterial strains (AB863, AB889, AB930, and AB960) exhibiting carbapenem resistance. They demonstrated high minimum inhibitory concentration (MIC) (128 mg/L) to meropenem and were categorized as extensively drug-resistant strains. Subsequently, they were identified as through 16S rRNA gene sequence analysis and species-specific PCR targeting the -like gene. Three strains were sequenced for their genomes to study the genetic determinants and functional relevance of carbapenem resistance. The draft genome length of the strains ranged from 3.8 to 4.0 Mbp. A total of 16 antibiotic resistance genes including the genes and which mediate carbapenem resistance were identified in the genomes. A comprehensive multilocus sequence typing analysis involving 95 strains from different Asian countries assigned the four strains to sequence type 2 (ST2), the most predominant ST circulating in Asia. Comparative genome analysis also revealed as the most dominant variant of -like gene and also a widespread distribution of gene. In addition, various mobile genetic elements associated with AMR genes and three efflux pumps families were detected in the genomes of the strains. Transformation of and genes resulted in meropenem resistance in the transformant which exhibited a MIC of 2 mg/L, thus confirming direct involvement of both genes in carbapenem resistance.
ISSN:2047-7732