Membrane Molecules Determined by the H-2 Associated Immune Response Region: Isolation and Some Properties

• Published in: Proceedings of the National Academy of Sciences - PNAS 1974-03, Vol.71 (3), p.648-652
• Main Authors: Cullen, S. E., David, C. S., Shreffler, D. C., Nathenson, S. G.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antigen-Antibody Complex; Antigen-Antibody Reactions; Antigens; Antigens - isolation & purification; Autoradiography; Biological Sciences: Immunology; Cell Membrane - immunology; Cell membranes; Cytotoxicity Tests, Immunologic; Detergents; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Epitopes; Gels; Genetic Linkage; Genotype; Histocompatibility; Histocompatibility Antigens; Immune Sera; Lymph nodes; Lymph Nodes - immunology; Lymphocytes; Mice; Mice, Inbred A; Mice, Inbred C57BL; Molecular Weight; Molecules; Precipitin Tests; Species Specificity; Spleen - immunology; Spleen cells; Tritium; Tumors
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.71.3.648

Membrane associated molecules that are probably glycoproteins could be specifically precipitated from NP-40 detergent solubilized extracts of radiolabeled mouse spleen or lymph node cells by antisera produced in congenic strain combinations differing only in the Ir gene region which is linked to the H-2 genes. These Ir region products were designated Lna (lymph node antigen) to conform to previous serological work. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unreduced specific immune precipitates revealed the presence of a possible dimer form, while reduced samples showed only a single peak equivalent to 30,000 daltons. Thus the Lna molecules are clearly distinct from the H-2D and H-2K molecules, which are about 45,000 daltons. Anti-Lna antibodies of different specificity can be present in a single serum; there were at least two separate antigen molecules present in one haplotype tested.