Cytochalasin B: Effects on Cell Morphology, Cell Adhesion, and Mucopolysaccharide Synthesis

Cytochalasin B reversibly causes extensive branching of myoblasts, fibroblasts, and nonencapsulated chondroblasts; it does not induce the formation of similar processes in myotubes, erythrocytes, amnion cells, encapsulated chondroblasts, or HeLa cells. The drug has no effect on the spontaneous contr...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1972-01, Vol.69 (1), p.253-257
Main Authors: Sanger, J. W., Holtzer, H.
Format: Article
Language:English
Subjects:
Amnion
Amnion - cytology
Animals
Biological Sciences: Zoology
Carbon Isotopes
Cell adhesion
Cell Adhesion - drug effects
Cell motility
Cells, Cultured
Chick Embryo
Chondrocytes
Connective Tissue - drug effects
Connective Tissue Cells
Cultured cells
Cytochalasins
Embryonic cells
Erythrocytes - drug effects
Glucosamine - pharmacology
Glycosaminoglycans
Glycosaminoglycans - biosynthesis
HeLa cells
HeLa Cells - drug effects
Muscle Contraction - drug effects
Muscle fibers
Muscle, Smooth - cytology
Mycotoxins - pharmacology
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Summary:Cytochalasin B reversibly causes extensive branching of myoblasts, fibroblasts, and nonencapsulated chondroblasts; it does not induce the formation of similar processes in myotubes, erythrocytes, amnion cells, encapsulated chondroblasts, or HeLa cells. The drug has no effect on the spontaneous contractions of isolated skeletal, cardiac, or smooth-muscle cells. Within 60 min, it depresses the incorporation of [14C]glucosamine into total mucopolysaccharide and glycoproteins by over 50%. The drug interferes with adhesion and sorting-out of dissociated embryonic cells. Cytochalasin B is likely to produce changes in components of the cell surface whose function is not readily or solely related to a system of ``primitive contractile microfilaments.''
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.1.253