AKR Murine Leukemia Virus Genome: Frequency of Sequences in DNA of High-, Low-, and Non-Virus-Yielding Mouse Strains

Studies with a single-stranded DNA probe complementary to the RNA of mouse-tropic AKR murine leukemia virus indicate that the complete genome of the AKR-type murine leukemia virus is present in the DNA of high- and low-virus-yielding mouse strains, while DNA of non-virus-yielding strains contains on...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-09, Vol.71 (9), p.3555-3559
Main Authors: Lowy, Douglas R., Chattopadhyay, Sisir K., Teich, Natalie M., Rowe, Wallace P., Levine, Arthur S.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological Sciences: Biochemistry
Cots
DNA
DNA probes
DNA, Single-Stranded
DNA, Viral - metabolism
Embryos
Genomes
Genotype
Hybridity
Kinetics
Leukemia Virus, Murine - metabolism
Mice
Mice, Inbred AKR
Murine leukemia virus
Nucleic Acid Hybridization
Phosphates
RNA, Viral
Temperature
Tritium
Virus Replication
Viruses
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Description
Summary:Studies with a single-stranded DNA probe complementary to the RNA of mouse-tropic AKR murine leukemia virus indicate that the complete genome of the AKR-type murine leukemia virus is present in the DNA of high- and low-virus-yielding mouse strains, while DNA of non-virus-yielding strains contains only a part of the genome. Furthermore, in those strains where the genome is complete, two populations of virus-specific DNA sequences can be identified (more abundant and less abundant species) according to their rate of association with the probe. Low-virus-yielding mouse strains contain fewer copies of the less abundant species and, consequently, fewer complete viral genomes than do high-virus-yielding strains. Thus, in the ten strains tested, there is a good correlation between completeness of the genome of AKR-type murine leukemia virus in cellular DNA and the capacity of the cells to release infectious AKR-type murine leukemia virus. Moreover, the number of complete viral genomes correlates with the frequency of infectious virus production by virus-positive strains. DNA from wild Mus musculus also contained viral sequences, the sample tested showing reassociation kinetics identical to the non-virus-producing strains.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.9.3555