Conformationally Dependent Low-Frequency Motions of Proteins by Laser Raman Spectroscopy

Low-frequency Raman bands (lower than 50 cm-1) exist in certain proteins. They are dependent upon the conformation of the protein molecule, but are relatively independent of the form of the sample, i.e., whether it is a film or a crystal. Low-frequency Raman spectra were obtained from samples of α -...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1972-06, Vol.69 (6), p.1467-1469
Main Authors: Brown, K. G., Erfurth, S. C., Small, E. W., Peticolas, W. L.
Format: Article
Language:English
Subjects:
Acylation
Chemical Phenomena
Chemistry
Chymotrypsin
Crystals
Deuterium
Infrared Rays
Lasers
Molecules
Physical Sciences: Chemistry
Polymers
Protein Conformation
Protein Denaturation
Proteins
Raman scattering
Scattering, Radiation
Single crystals
Sodium
Sodium Dodecyl Sulfate
Spectrum Analysis
Sulfates
Vibration
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Summary:Low-frequency Raman bands (lower than 50 cm-1) exist in certain proteins. They are dependent upon the conformation of the protein molecule, but are relatively independent of the form of the sample, i.e., whether it is a film or a crystal. Low-frequency Raman spectra were obtained from samples of α -chymotrypsin that had been prepared in several ways. A peak at about 29 cm-1was found for all samples except the one that had been denatured with sodium dodecyl sulfate. Such low frequency motions must arise from vibrations that involve all, or very large portions, of the protein molecule.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.6.1467