Restoration of Hypoxanthine Phosphoribosyl Transferase Activity in Mouse 1R Cells after Fusion with Chick-Embryo Fibroblasts

Fusion of the 1R mouse cell, which lacks activity of hypoxanthine phosphoribosyl transferase (EC 2.4.2.8), with chick-embryo fibroblasts yielded progeny cells that survived in hypoxanthine-aminopterin-thymidine selective medium. This property and the failure of the progeny to survive in 8-azaguanine...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1973-07, Vol.70 (7), p.1998-2002
Main Authors: Bakay, Bohdan, Croce, Carlo M., Koprowski, Hilary, Nyhan, William L.
Format: Article
Language:English
Subjects:
Adenine
Aminopterin
Animals
Biological Sciences: Cell Biology
Carbon Isotopes
Cell Fusion
Cell Line
Cell lines
Cells
Chick Embryo
Chromosomes
Clone Cells
Culture Media
Electrophoresis, Polyacrylamide Gel
Embryonic cells
Enzyme Activation
Enzymes
Fibroblasts
Glucosephosphate Dehydrogenase - analysis
Hybrid Cells - enzymology
Hypoxanthines
Hypoxia
Karyotyping
L cells
L Cells (Cell Line)
Mice
Pentosyltransferases - analysis
Pentosyltransferases - biosynthesis
Somatic cells
Thymidine
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Summary:Fusion of the 1R mouse cell, which lacks activity of hypoxanthine phosphoribosyl transferase (EC 2.4.2.8), with chick-embryo fibroblasts yielded progeny cells that survived in hypoxanthine-aminopterin-thymidine selective medium. This property and the failure of the progeny to survive in 8-azaguanine indicated that hypoxanthine phosphoribosyl transferase activity was present. Electrophoretic analysis revealed that the enzyme was of mouse, not chick, origin. These observations are consistent with the operation of a regulator gene responsible for the absence of hypoxanthine phosphoribosyl-transferase activity in the 1R cell and its presence in the progeny.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.70.7.1998