Manipulation of the Phospholipid Composition of Tissue Culture Cells

Methods have been devised to significantly alter the phospholipid composition of LM cells grown in serum-free tissue culture medium. The polar head groups of the phospholipids, as well as the acyl groups of these lipids, can be changed. When LM cells were grown in medium containing choline analogues...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-10, Vol.71 (10), p.4072-4076
Main Authors: Glaser, Michael, Ferguson, Karen A., Vagelos, P. Roy
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological Sciences: Biochemistry
Cell growth
Cell Membrane - metabolism
Cells, Cultured - metabolism
Chemical composition
Choline - analogs & derivatives
Choline - metabolism
Chromatography
Chromatography, Thin Layer
Culture Media
Cultured cells
Fatty acids
Fatty Acids - analysis
Flasks
L Cells (Cell Line)
Linoleic Acids - metabolism
Lipids
Mice
Phosphatidylethanolamines - biosynthesis
Phospholipids
Phospholipids - analysis
Phospholipids - metabolism
Phosphorus Radioisotopes
Tissue culture techniques
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Methods have been devised to significantly alter the phospholipid composition of LM cells grown in serum-free tissue culture medium. The polar head groups of the phospholipids, as well as the acyl groups of these lipids, can be changed. When LM cells were grown in medium containing choline analogues, either N-methylethanolamine or N,N-dimethylethanolamine, or the unnatural analogues, 1-2-amino-l-butanol or 3-amino-l-propanol, up to 50% of the cellular phospholipids contained the analogue supplied. When linoleate was added to the cells as a bovine serum albumin complex, up to 40% of the fatty acids of the phospholipids were linoleate. Under the conditions discussed, the polar head group composition, the fatty acid composition, or both together could be varied in the membrane phospholipids.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.10.4072