Correlation between hydrolysis of the -lactam bond of the cephalosporin nucleus and expulsion of the 3-substituent

The hydrolysis of two cephalosporins by three different beta-lactamases has been studied. Each enzyme caused a decrease in ultraviolet absorption, a loss of biological activity, and the release of the leaving group from the 3-position. The changes occurred at the same rate and to the same extent wit...

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Bibliographic Details
Published in:Journal of bacteriology 1972-06, Vol.110 (3), p.988
Main Authors: O'Callaghan, C H, Kirby, S M, Morris, A, Waller, R E, Duncombe, R E
Format: Article
Language:English
Subjects:
Absorption
Autoanalysis
Azides - biosynthesis
beta-Lactams - metabolism
Cephalosporins - metabolism
Chemical Phenomena
Chemistry
Chromatography, Ion Exchange
Enterobacter - enzymology
Enterobacteriaceae - enzymology
Escherichia coli - enzymology
Hydrolysis
Klebsiella - enzymology
Penicillinase - isolation & purification
Penicillinase - metabolism
Pyridines - biosynthesis
Spectrophotometry
Ultraviolet Rays
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Summary:The hydrolysis of two cephalosporins by three different beta-lactamases has been studied. Each enzyme caused a decrease in ultraviolet absorption, a loss of biological activity, and the release of the leaving group from the 3-position. The changes occurred at the same rate and to the same extent with each enzyme, and it is inferred that the loss of the leaving group is a consequence of, and not a prerequisite for, hydrolysis of the beta-lactam ring.
ISSN:0021-9193