Antitumor Effects of Antibody—Diphtheria Toxin Conjugates. III. Cyclophosphamide-Induced Immune Unresponsiveness to Conjugates

Of 9 hamsters immunized with diphtheria toxin-hamster γ-globulin conjugates and rechallenged with this antigen 15 days later, 8 exhibited neutralizing antibodies against the conjugates 22 days after the initial immunization. Hamsters identically immunized, but also given cyclophos-phamide (CY) 20 ho...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1975-09, Vol.55 (3), p.709-712
Main Authors: Moolten, Frederick L., Capparell, Neva J., Zajdel, Shirley H.
Format: Article
Language:English
Subjects:
Animals
Antibody Formation - drug effects
Cricetinae
Cyclophosphamide - pharmacology
Depression, Chemical
Diphtheria Toxin
Female
gamma-Globulins
Immunization
Neutralization Tests
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Summary:Of 9 hamsters immunized with diphtheria toxin-hamster γ-globulin conjugates and rechallenged with this antigen 15 days later, 8 exhibited neutralizing antibodies against the conjugates 22 days after the initial immunization. Hamsters identically immunized, but also given cyclophos-phamide (CY) 20 hours after the first immunization, had a much lower incidence of positive responses (2/10) and lower antibody levels in the positive responders. After further immunization with the conjugates, all CY-treated hamsters eventually developed neutralizing antibodies, but exhibited a reduction in antibody levels after a second exposure to CY. Hamsters immunized with diphtheria toxoid developed antibodies that were effective in neutralizing diphtheria toxin, but less effective against the diphtheria toxin conjugates.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/55.3.709