An Improved Cell Volume Analyzer

A cell volume sensing instrument has been built which employs the principle used in the Coulter counter in which a cell changes the impedance of a narrow orifice as it passes through the orifice. An improved transducer utilizes a coaxial flow of the cell suspension inside a sheath of cell‐free solut...

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Bibliographic Details
Published in:Review of scientific instruments 1971-08, Vol.42 (8), p.1157-1163
Main Authors: Merrill, J. T., Veizades, N., Hulett, H. R., Wolf, P. L., Herzenberg, L. A.
Format: Article
Language:English
Subjects:
Blood Cell Count
Blood Cells
Humans
Methods
Reticulocytes
Transducers
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Summary:A cell volume sensing instrument has been built which employs the principle used in the Coulter counter in which a cell changes the impedance of a narrow orifice as it passes through the orifice. An improved transducer utilizes a coaxial flow of the cell suspension inside a sheath of cell‐free solution through the orifice, thereby avoiding some drawbacks of earlier systems. The instrument is described, and the procedure necessary to achieve acceptable operation is discussed. The output for normal human red blood cells is nearly symmetrical with a narrow peak. Abnormal blood samples show various departures from symmetry and narrowness of peak. The output of this instrument is compared with that from a commercially available instrument, and our data show a more accurate representation of the actual distribution of blood volumes. The resolution of this instrument is such that it could be of significant value in a clinical laboratory.
ISSN:0034-6748
1089-7623
DOI:10.1063/1.1685332