Interallelic Complementation in Hybrid Cells Derived from Human Diploid Strains Deficient in Galactose-1-Phosphate Uridyl Transferase Activity

Galactose-1-phosphate uridyl transferase activity has been demonstrated in hybrid cells formed from the fusion of human diploid fibroblasts obtained from different patients with galactosemia. The enzyme produced by the hybrid cells was similar to the normal enzyme in regard to Km, pH optimum, and el...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1970-10, Vol.67 (2), p.976-982
Main Authors: Nadler, Henry L., Chacko, Claramma M., Rachmeler, Martin
Format: Article
Language:English
Subjects:
Alleles
Biological Sciences: Genetics
Cell Fusion
Complementation
Diploidy
Electrophoresis
Enzymes
Fibroblasts
Galactosemias - enzymology
Galactosemias - genetics
Gels
Genetic Complementation Test
Humans
Hybrid cells
Hybridity
Hybridization, Genetic
Hydrogen-Ion Concentration
Nucleotidyltransferases - analysis
Starches
Thermal stability
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Description
Summary:Galactose-1-phosphate uridyl transferase activity has been demonstrated in hybrid cells formed from the fusion of human diploid fibroblasts obtained from different patients with galactosemia. The enzyme produced by the hybrid cells was similar to the normal enzyme in regard to Km, pH optimum, and electrophoretic mobility on starch gel, but differed in specific activity, Vmax and thermal stability. This is the first reported example of interallelic complementation in fused human diploid cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.67.2.976