RNA Transcripts for I-J Polypeptides are Apparently not Encoded between the I-A and I-E Subregions of the Murine Major Histocompatibility Complex

The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been ma...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-09, Vol.80 (18), p.5704-5708
Main Authors: Kronenberg, Mitchell, Steinmetz, Michael, Kobori, Joan, Kraig, Ellen, Kapp, Judith A., Pierce, Carl W., Sorensen, Craig M., Suzuki, Gen, Tada, Tomio, Hood, Leroy
Format: Article
Language:English
Subjects:
Animals
Cosmids
DNA
DNA probes
Exons
Genes
Genetic mapping
Hybridomas
Hybridomas - analysis
Major Histocompatibility Complex
Mice
Mice, Inbred BALB C
Molecules
Nucleic Acid Hybridization
RNA
RNA - analysis
T lymphocytes
T-Lymphocytes, Regulatory - analysis
Transcription, Genetic
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Summary:The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.18.5704