Antitumor Antibody Synthesis In Vitro Induced in Mouse Spleen Cells by Xenogeneic Immune RNA

• Published in: JNCI : Journal of the National Cancer Institute 1978-03, Vol.60 (3), p.599-603
• Main Authors: Kern, David H., Pilch, Yosef H.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Neoplasm - biosynthesis; Antibody Specificity; Cytotoxicity Tests, Immunologic; Female; Guinea Pigs; In Vitro Techniques; Mammary Neoplasms, Experimental - immunology; Mice; Mice, Inbred C3H; RNA - immunology; Sarcoma, Experimental - immunology; Spleen - immunology
• ISSN: 0027-8874; 1460-2105
• DOI: 10.1093/jnci/60.3.599

Globulins derived from culture medium and cell lysates that had been prepared from normal, nonimmune C3H mouse spleen cells, following incubation with xenogeneic immune RNA (I-RNA), contained antitumor antibody activity. Globulins derived from spleen cells incubated with I-RNA extracted from the lymphoid organs of guinea pigs that had been immunized with either of two cross-reacting chemically induced C3H mouse sarcomas reacted against target cells of both tumors, but not against target cells derived from a spontaneously arising syngeneic osteosarcoma. Globulins obtained from spleen cells that had been incubated with I-RNA from guinea pigs immunized either with osteosarcoma cells or cells from a spontaneous C3H mouse mammary tumor reacted with target cells from either of the two chemically induced sarcomas, although globulins from spleen cells that had been incubated with antlosteosarcoma I- RNA did not react with osteosarcoma target cells. Globulins derived from spleen cells incubated with I-RNA extracted from guinea pigs that had been immunized with either complete Freund's adjuvant only or with a pool of normal C3H mouse tissues exhibited no reactivity against any of the three target cells tested. The antitumor antibodies whose synthesis was induced by xenogeneic I-RNA were apparently specific for the particular tumor used to immunize the I-RNA donor.