Adenovirus 2 Early Gene Expression Promotes Susceptibility to Effector Cell Lysis of Hybrids Formed between Hamster Cells Transformed by Adenovirus 2 and Simian Virus 40

Weakly oncogenic adenovirus 2 (Ad2)-transformed LSH hamster cells are sensitive to lysis by spontaneously cytolytic lymphoid cells and activated macrophages, whereas highly oncogenic simian virus 40 (SV40)-transformed LSH cells are relatively resistant to these nonspecific effector cells. Somatic ce...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-10, Vol.80 (19), p.5995-5999
Main Authors: Cook, James L., Hauser, Janet, Patch, Cephas T., Lewis, Andrew M., Levine, Arthur S.
Format: Article
Language:English
Subjects:
Adenoviruses, Human - genetics
Adenoviruses, Human - immunology
Animals
Antigens, Viral - genetics
Cell Line
Cell lines
Cell Transformation, Neoplastic
Cellular immunity
Cricetinae
Cytotoxicity, Immunologic
Disease Susceptibility
Embryo, Mammalian
Embryonic cells
Genes, Viral
Hybrid cells
Hybridity
Lymphocytes
Macrophages - immunology
Mesocricetus
Simian virus 40 - genetics
Simian virus 40 - immunology
Somatic cells
Spleen cells
Transformed cell line
Tumor cell line
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Weakly oncogenic adenovirus 2 (Ad2)-transformed LSH hamster cells are sensitive to lysis by spontaneously cytolytic lymphoid cells and activated macrophages, whereas highly oncogenic simian virus 40 (SV40)-transformed LSH cells are relatively resistant to these nonspecific effector cells. Somatic cell hybrids formed between Ad2- and SV40-transformed hamster cells, which expressed Ad2 tumor (T) antigens, exhibited an increased cytolytic susceptibility compared to Ad2 T antigen-negative cell hybrids or nonhybrid SV40-transformed cells. No correlation was found between the expression of SV40 T antigen in hybrid cells and cytolytic susceptibility. The results suggest the existence of a novel function for early Ad2 genome-encoded polypeptides (T antigens) expressed in transformed hamster cells--the induction of susceptibility to destruction mediated by immunologically non-specific effector cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.19.5995