Detection of putative adduct with fluorescence characteristics identical to 2,3-dihydro-2-(7′-guanyl)-3-hydroxyaflatoxin B1 in human urine collected in Murang'a district, Kenya

Food samples collected in Murang'a district, Kenya are known to be contaminated with a mycotoxin, aflatoxin B1 (AFB), and a positive correlation exists between the dietary intake of AFB and the incidence of liver cancer. When urine samples collected in this district were analyzed for the presen...

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Bibliographic Details
Published in:Carcinogenesis (New York) 1983-09, Vol.4 (9), p.1193-1195
Main Authors: Autrup, Herman, Bradly, Katherine A., Shamsuddin, A.K.M., Wakhisi, Johnston, Wasunna, A.
Format: Article
Language:English
Subjects:
Aflatoxin B1
Aflatoxins - metabolism
Aflatoxins - urine
Biological and medical sciences
Biotransformation
Carcinogenesis, carcinogens and anticarcinogens
Chemical agents
Guanine - analogs & derivatives
Guanine - urine
Humans
Kenya
Medical sciences
Spectrometry, Fluorescence - methods
Tumors
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Summary:Food samples collected in Murang'a district, Kenya are known to be contaminated with a mycotoxin, aflatoxin B1 (AFB), and a positive correlation exists between the dietary intake of AFB and the incidence of liver cancer. When urine samples collected in this district were analyzed for the presence of 2,3-dihydro-2-(7′-guanyl)-3-hydroxyaflatoxin B1 (AFB-Gual) by h.p.l.c, 6 of 81 samples had a detectable level of a compound whose fluorescence spectrum was identical to chemically synthesized AFB-Gual as confirmed by photon-counting fluorescence spectrophotometry. These results are an indication of interaction between the ultimate carcinogenic form of AFB and cellular nucleic acids in vivo and further support the hypothesis that AFB may play an important role in the etiology of human liver cancer.
ISSN:0143-3334
1460-2180
DOI:10.1093/carcin/4.9.1193