Growth, Metastasis, Immunogenicity, and Chromosomal Content of a Nickel-Induced Rhabdomyosarcoma and Subsequent Cloned Cell Lines in Rats

• Published in: JNCI : Journal of the National Cancer Institute 1983-12, Vol.71 (6), p.1241-1245
• Main Authors: Pot-Deprun, J., Poupon, M-F., Sweeney, F. L., Chouroulinkov, I.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Chemical agents; Chromosomes - ultrastructure; Clone Cells - immunology; Clone Cells - ultrastructure; Immunization; Karyotyping; Lymphatic Metastasis; Male; Medical sciences; Neoplasm Transplantation; Nickel - pharmacology; Rats; Rats, Inbred Strains; Rhabdomyosarcoma - chemically induced; Rhabdomyosarcoma - physiopathology; Tumors
• ISSN: 0027-8874; 1460-2105
• DOI: 10.1093/jnci/71.6.1224

Karyotype patterns, growth, metastasis, and immunogenicity were compared in a rhabdomyosarcoma induced by a single injection of metallic nickel with those in subsequent tumor-cloned cell lines. The primary tumor was induced in a male WAG rat by im injection of 20 mg nickel powder. The parental cell line (9–4/0) and 8 cell subpopulations (J 9–4) isolated from a primary tumor by cloning on agarose were examined. The tumor cell dose inducing tumors in 50% of the animals after sc injection (TD50) and the in vitro growth characteristics showed a marked heterogeneity between parental and cloned tumor cell lines. In vitro doubling times and saturation densities were also heterogeneous without showing a discernible relationship with TD5o. Chromosome patterns of the cell lines exhibited very similar modal numbers, whereas chromosome numbers were somewhat different; neither of these exhibited any correlation with tumorigenicity. Parental cell line 9–4/0 expressed a significant degree of immunogenicity, but it did not protect against pulmonary metastasis in immunized rats. Among 6 clones studied, only clone J 9– 4/2 appeared to be immunogenic and reduced metastatic spread. The relevance of the comparison between the different characteristics is discussed.