O6-methylguanine removal by competent and incompetent human lymphoblastoid lines from the same male individual

Two cell lines, one proficient (Mex+) and one deficient (Mex-) in the ability to remove O6-methylguanine, have been isolated by Epstein-Barr virus-mediated transformation of a single blood sample obtained from a normal human male. Extracts of untreated cells differ in their O6-methylguanine transfer...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1983-07, Vol.43 (7), p.3316-3320
Main Authors: SKLAR, R. M, STRAUSS, B. S
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - isolation & purification
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Cell Division - drug effects
Cell Line
Chemical agents
Chromosome Aberrations
Guanine - analogs & derivatives
Guanine - isolation & purification
Humans
Karyotyping
Lymphocyte Activation
Lymphocytes - metabolism
Male
Medical sciences
Methylnitronitrosoguanidine - pharmacology
Tumors
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Summary:Two cell lines, one proficient (Mex+) and one deficient (Mex-) in the ability to remove O6-methylguanine, have been isolated by Epstein-Barr virus-mediated transformation of a single blood sample obtained from a normal human male. Extracts of untreated cells differ in their O6-methylguanine transferase (methyl acceptor protein) activity. Although both lines arise from the same individual, they show great difference in their sensitivities to the toxic action of N-methyl-N'-nitro-N-nitrosoguanidine. Chromosome counts of the strains reveal a modal number of 46 for both. Neither X-inactivation nor a gross abnormality in chromosome number can be the cause of the difference between the two lines.
ISSN:0008-5472
1538-7445