Complement Synthesis by Guinea Pig Peritoneal Macrophages: Failure To Detect Chemical Carcinogens

In vitro production of the second and fourth components of complement (C2 and C4. respectively) by peritoneal macrophages from noninbred Hartley guinea pigs was tested after the animals had been inoculated with known carcinogens. The system demonstrated the capacity of N-nitrosodimethylamine to decr...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1981-01, Vol.66 (1), p.141-145
Main Authors: Kunke, Kathleen S., Strunk, Robert C.
Format: Article
Language:English
Subjects:
Animals
Body Weight - drug effects
Carcinogens - pharmacology
Carcinogens - toxicity
Complement C2 - analysis
Complement C2 - immunology
Complement C4 - analysis
Complement C4 - immunology
Complement System Proteins - analysis
Complement System Proteins - immunology
Dimethylnitrosamine - pharmacology
Drug Evaluation, Preclinical
Female
Guinea Pigs
Immune Adherence Reaction
Lethal Dose 50
Macrophages - analysis
Macrophages - drug effects
Macrophages - immunology
Male
Peritoneum - drug effects
Peritoneum - immunology
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Summary:In vitro production of the second and fourth components of complement (C2 and C4. respectively) by peritoneal macrophages from noninbred Hartley guinea pigs was tested after the animals had been inoculated with known carcinogens. The system demonstrated the capacity of N-nitrosodimethylamine to decrease C2 and C4 production. However, a similar decrease in C2 and C4 production was seen with only CCI4 1 of the 10 chemical carcinogens studied. This system had little usefulness as a short-term screening procedure for the detection of carcinogenicity. The effect of the carcinogens on several other functions of peritoneal macrophages was also determined. The number of peritoneal exudate cells (PEC) was significantly lower in carcinogen-inoculated animals than in solvent-inoculated controls for three carcinogens: BeS04, P
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/66.1.141