Serum Contains a Platelet-Derived Transforming Growth Factor

High concentrations of fetal bovine serum induced colony formation in soft agar by anchorage-dependent, nontransformed mouse AKR-2B and rat NRK cells. The colony-stimulating activity in fetal bovine serum was precipitated by 45% saturated ammonium sulfate and migrated in molecular sieve chromatograp...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-09, Vol.79 (17), p.5312-5316
Main Authors: Childs, Chris Bjornson, Proper, Jacqueline A., Tucker, Ronald F., Moses, Harold L.
Format: Article
Language:English
Subjects:
Animals
Biological Assay
Blood Platelets - analysis
Cattle
Cell Division - drug effects
Cell growth
Cell Line
Cell lines
Cells
Chromatography
Fetal Blood - analysis
Fetus
Growth Substances - isolation & purification
Growth Substances - pharmacology
Humans
Insulin
Molecular weight
Peptides - isolation & purification
Peptides - pharmacology
Platelet-Derived Growth Factor
Platelets
Quaternary ammonium compounds
Sulfates
Ungulates
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Summary:High concentrations of fetal bovine serum induced colony formation in soft agar by anchorage-dependent, nontransformed mouse AKR-2B and rat NRK cells. The colony-stimulating activity in fetal bovine serum was precipitated by 45% saturated ammonium sulfate and migrated in molecular sieve chromatography as a single peak of activity in the 10,000-15,000 molecular weight range. The colony-stimulating activity was heat and acid stable and was destroyed by trypsin and dithiothreitol, indicating the activity is due to a polypeptide that requires disulfide bonds for biological activity. No competition for binding to the epidermal growth factor receptor was associated with the colony-stimulating activity. Isoelectric focusing revealed activity in the pI 4--5 range. The colony-stimulating activity in serum appeared to be of platelet origin because platelet-poor plasma and platelet-poor plasma-derived serum contained little activity, whereas acid/ethanol extracts of bovine and human platelets had potent colony-stimulating activity. Chromatography of platelet extracts on Bio-Gel P-60 revealed peaks of AKR-2B colony-stimulating activity in the 12,000 and 20,000 molecular weight ranges. The other biological and chemical properties of the platelet colony-stimulating activity were the same as those for the serum activity. The data indicate the presence in serum of a platelet-derived growth factor(s) with properties similar to those of the transforming growth factors.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.17.5312