Formation of Symbiotic Complex by Microenvironment-Dependent Mouse Leukemias and Thymic Epithelial Reticular Cells

Developing thymic leukemias of the mouse have been assumed to form symbiotic complexes with thymic microenvironments. This symbiosis is morphologically based on pseudoemperipolesis (PEMP). The mechanism of the association of microenvironment-dependent leukemia cells with thymic epithelial reticular...

Full description

Saved in:
Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1982-09, Vol.69 (3), p.627-637
Main Authors: Nishi, Yoshimi, Yoshikawa, Kazuhiro, Hiai, Hiroshi, Notake, Kunihiro, Shisa, Hayase, Nishizuka, Yasuaki
Format: Article
Language:English
Subjects:
Animals
Cell Communication
Cell Line
Cell Movement - drug effects
Colchicine - pharmacology
Cytochalasins - pharmacology
Cytoskeleton - ultrastructure
Dose-Response Relationship, Drug
Epithelial Cells
Epithelium - pathology
Leukemia, Experimental - pathology
Mice
Mice, Inbred AKR
Microscopy, Electron, Scanning
Microscopy, Phase-Contrast
Symbiosis - drug effects
Tetradecanoylphorbol Acetate - pharmacology
Thymus Gland - cytology
Thymus Gland - pathology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Developing thymic leukemias of the mouse have been assumed to form symbiotic complexes with thymic microenvironments. This symbiosis is morphologically based on pseudoemperipolesis (PEMP). The mechanism of the association of microenvironment-dependent leukemia cells with thymic epithelial reticular cells (TER) was analyzed in vitro by scanning electron microscopy, microcinematography, and a quantitative assessment of PEMP. PEMP was a consequence of active locomotion of the leukemia cells, with TER passively accepting the leukemia cells' “crawling” under their cytoplasm. The integrity of the cytoskeletal system of both cells was essentially required for PEMP, since cytochalasins and colchicine were highly inhibitory to PEMP. The mechanism of action of these compounds was probably dual: inhibition of the locomotive movements of the leukemia cells. A similar inhibition of PEMP was also observed with the tumor promoter 1 2-O-tetradecanoylphorbol 13-acetate.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/69.3.627