Studies on Human Proinsulin C-peptide Radioimmunoassay Method: Preparation of Antiserum and Its Specificity

The antisera using at final dilution of 1 : 10, 000 have been prepared by immunizing synthetic human proinsulin connecting peptide to rabbits for human proinsulin C-peptide radioimmunoassay. The cross reactivities of human proinsulin C-peptide derivatives with the prepared antisera were reduced by l...

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Bibliographic Details
Published in:RADIOISOTOPES 1977/05/15, Vol.26(5), pp.291-295
Main Authors: NAKAZAWA, Nobuhiko, MAKI, Kentaro, OGAWA, Hiroshi, IKEDA, Osamu, SAKAGAMI, Masanori, SAKURA, Naoki, YANAIHARA, Noboru
Format: Article
Language:Japanese
Subjects:
C-Peptide - analysis
Cross Reactions
Epitopes
Humans
Immune Sera
Immunologic Techniques
Peptides - analysis
Radioimmunoassay - methods
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Summary:The antisera using at final dilution of 1 : 10, 000 have been prepared by immunizing synthetic human proinsulin connecting peptide to rabbits for human proinsulin C-peptide radioimmunoassay. The cross reactivities of human proinsulin C-peptide derivatives with the prepared antisera were reduced by leaving amino acid residues from N terminal, although this phenomenon was a little different among antisera. Those results suggested that main antigen determinant in N terminal 31-38 of human proinsulin connecting peptide. The cross reactivities of other animal proinsulin C-peptide and other peptide hormones with the prepared antisera were not recognized at 103 p mole/ml.
ISSN:0033-8303
1884-4111
DOI:10.3769/radioisotopes.26.5_291