Organotypic growth and differentiation of human mammary gland in sponge-gel matrix supported histoculture

Blocks of breast tissue obtained during radical mastectomies from 23 patients with mammary gland carcinomas were used for cultivation in native-state, gel-supported histocultures. We show that the human mammary gland can be successfully maintained in this system so that normal epithelial breast stru...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 1994-08, Vol.30A (8), p.490
Main Authors: Baibakov, B A, Chipisheva, T A, Guelstein, V I, Ermilova, V D, Polevaya, E B, Vasiliev, J M, Margolis, L B
Format: Article
Language:English
Subjects:
Adult
Aged
Breast - growth & development
Breast - metabolism
Breast Neoplasms - pathology
Culture Techniques - methods
Epithelium - growth & development
Epithelium - metabolism
Female
Humans
Immunohistochemistry
Keratins - metabolism
Middle Aged
Time Factors
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Summary:Blocks of breast tissue obtained during radical mastectomies from 23 patients with mammary gland carcinomas were used for cultivation in native-state, gel-supported histocultures. We show that the human mammary gland can be successfully maintained in this system so that normal epithelial breast structures proliferate and undergo differentiation for several weeks and a well-developed system of ducts and lobules is formed. Using antibodies to individual keratins 17 and 8 we have shown for the first time that ducts and alveoles developing in vitro undergo differentiation into the lining epithelium and myoepithelium in the same way as mammary gland epithelium in vivo. Growth of epithelial structures in vitro is also accompanied by the development of continuous basal membrane.
ISSN:1071-2690