Detection of Mycoplasma hyopneumoniae in nose swabs from pigs by in vitro amplification of the 16S rRNA gene

We report the use of PCR to detect DNA from Mycoplasma hyopneumoniae, the etiological agent of enzootic porcine pneumonia. A primer set was designed for the amplification of a 649-bp fragment of the 16S rRNA gene from M. hyopneumoniae. The PCR product was identified by ethidium bromide staining afte...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 1995-04, Vol.33 (4), p.893-897
Main Authors: Mattsson, J G, Bergström, K, Wallgren, P, Johansson, K E
Format: Article
Language:English
Subjects:
Animal bacterial diseases
Animals
Bacterial diseases
Base Sequence
Biological and medical sciences
cerdo
DNA Primers - genetics
gene
genes
Genes, Bacterial
In Vitro Techniques
Infectious diseases
lungs
Medical sciences
Molecular Sequence Data
Mycoplasma - genetics
Mycoplasma - isolation & purification
Mycoplasma hyopneumoniae
nariz
Nasal Mucosa - microbiology
nez
nose
pcr
Pneumonia of Swine, Mycoplasmal - diagnosis
Pneumonia of Swine, Mycoplasmal - microbiology
Pneumonia of Swine, Mycoplasmal - veterinary
Polymerase Chain Reaction - statistics & numerical data
porcin
poumon
pulmones
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sensitivity and Specificity
swine
Swine - microbiology
Swine Diseases - diagnosis
Swine Diseases - microbiology
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Description
Summary:We report the use of PCR to detect DNA from Mycoplasma hyopneumoniae, the etiological agent of enzootic porcine pneumonia. A primer set was designed for the amplification of a 649-bp fragment of the 16S rRNA gene from M. hyopneumoniae. The PCR product was identified by ethidium bromide staining after gel electrophoresis and by Southern hybridization with an M. hyopneumoniae-specific oligonucleotide probe. No amplification was observed from any other porcine bacteria tested, including several closely related mycoplasmas. It was also possible to demonstrate the presence of M. hyopneumoniae in bronchial ravage samples and lung tissue samples from experimentally infected pigs. Furthermore, the PCR system was used for analysis of nasal samples obtained from pigs in a fattening herd. By this method, we were able to detect M. hyopneumoniae in nose swabs from naturally infected pigs. However, our results suggest that M. hyopneumoniae can be detected in the nasal cavities only during a limited time period.
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.33.4.893-897.1995