Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase

The gene encoding extracellular xylanase (xynA) was amplified as a 770 bp DNA fragment from Bacillus subtilis 168 chromosomal DNA by PCR. The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 1995-02, Vol.141, p.281-290
Main Authors: Wolf, M, Geczi, A, Simon, O, Borriss, R
Format: Article
Language:English
Subjects:
Bacillus subtilis - enzymology
Bacillus subtilis - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Biological Sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Cellulase - deficiency
Cellulase - genetics
Cloning, Molecular
Endo-1,4-beta Xylanases
Fundamental and applied biological sciences. Psychology
genbank/z29076
genbank/z34519
genbank/z46862
Genes, Bacterial - genetics
Genetic Complementation Test
Genetic Linkage
Genetics
Glucans - metabolism
Glycoside Hydrolases - deficiency
Glycoside Hydrolases - genetics
Hydrolysis
Microbiology
Mission oriented research
Molecular Sequence Data
Mutagenesis
Restriction Mapping
Sequence Analysis, DNA
Xylans - metabolism
Xylosidases - deficiency
Xylosidases - genetics
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Summary:The gene encoding extracellular xylanase (xynA) was amplified as a 770 bp DNA fragment from Bacillus subtilis 168 chromosomal DNA by PCR. The genes encoding endo-beta-1,4glucanase (eglS) and endo-beta-1,3-1,4 glucanase (bglS) were isolated from a genomic library of B. subtilis 168. The sequences of xynA and eglS were identical to those of the xylanase and cellulase genes from B. subtilis PAP115. Integrative plasmids containing DNA fragments with deletions in the coding region of the genes were constructed and used to replace the chromosomal eglS, bglS and xynA genes of B. subtilis 168. Strains without any detectable activity against xylan (Xyn-), carboxymethylcellulose (Egl-) or mixed linked beta-1,3-1,4-glucan (Egl- Bgl-) were obtained. The genes were mapped at 170 degrees (eglS), 175 degrees (xynA) and 340 degrees (bglS) on the B. subtilis chromosome.
ISSN:1350-0872
1465-2080
DOI:10.1099/13500872-141-2-281