Manual and automated determination of 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil and its metabolite (E)-5-(2-bromovinyl)uracil in urine

This paper describes the determination of 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil in urine. The method involves sample clean-up by liquid-liquid extraction with ethyl acetate followed by high-performance liquid chromatographic (HPLC) analysis. The sample preparation may be performed eithe...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1995-02, Vol.664 (2), p.357
Main Authors: Whigan, D B, Schuster, A E
Format: Article
Language:English
Subjects:
Antiviral Agents - pharmacokinetics
Antiviral Agents - urine
Arabinofuranosyluracil - analogs & derivatives
Arabinofuranosyluracil - pharmacokinetics
Arabinofuranosyluracil - urine
Autoanalysis
Bromouracil - analogs & derivatives
Bromouracil - urine
Chromatography, High Pressure Liquid
Humans
Robotics
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Summary:This paper describes the determination of 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil in urine. The method involves sample clean-up by liquid-liquid extraction with ethyl acetate followed by high-performance liquid chromatographic (HPLC) analysis. The sample preparation may be performed either manually or automatically using a Zymark Py-robotic system. The chloro analog of the parent compound, CV-araU, is used as the internal standard. As low as 0.1 microgram of analyte per ml of urine can be measured. This sensitivity is adequate for pharmacokinetic studies but could be improved quite easily if necessary.
ISSN:1572-6495
DOI:10.1016/0378-4347(94)00487-P