Spermatogenesis Following Male Germ-Cell Transplantation

In the adult male, a population of diploid stem-cell spermatogonia continuously undergoes self-renewal and produces progeny cells, which initiate the complex process of cellular differentiation that results in mature spermatozoa. We report here that stem cells isolated from testes of donor male mice...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1994-11, Vol.91 (24), p.11298-11302
Main Authors: Brinster, R L, Zimmermann, J W
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Busulfan
Cellular biology
Embryonic stem cells
Germ cells
Infertility, Male - pathology
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Microinjections
Pipettes
Rodents
Seminiferous tubules
Seminiferous Tubules - cytology
Spermatogenesis
Spermatogonia
Spermatogonia - transplantation
Spermatozoa
Stem Cell Transplantation
Stem cells
Testes
Testis - cytology
Totipotent stem cells
Transplants & implants
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Summary:In the adult male, a population of diploid stem-cell spermatogonia continuously undergoes self-renewal and produces progeny cells, which initiate the complex process of cellular differentiation that results in mature spermatozoa. We report here that stem cells isolated from testes of donor male mice will repopulate sterile testes when injected into seminiferous tubules. Donor cell spermatogenesis in recipient testes showed normal morphological characteristics and produced mature spermatozoa. This methodology, besides opening new avenues of basic research into spermatogenesis and stem-cell self-renewal, may prove useful as a tool for biomedical science and biotechnology.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.24.11298