Distribution of 111in‐ and 125i‐labeled monoclonal antibody 17‐1a in mice bearing xenografts of human pancreatic carcinoma hup‐t4

• Published in: Cancer 1994-02, Vol.73 (S3), p.800-807
• Main Authors: Maeda, Masatoshi, Shoji, Miki, Kawagoshi, Toshiyuki, Futatsuya, Ryusuke, Honda, Takashi, Brady, Luther W.
• Format: Article
• Language: English
• Subjects: Absorption; Animals; Antibodies, Monoclonal - metabolism; Biological and medical sciences; Diseases of the digestive system; Humans; Immunotoxins - metabolism; indium radioisotopes; Indium Radioisotopes - metabolism; iodine radioisotopes; Iodine Radioisotopes - metabolism; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Nude; monoclonal antibodies; Neoplasm Transplantation; pancreatic neoplasms; Pancreatic Neoplasms - metabolism; radioimmunodetection; radioimmunotherapy; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Tissue Distribution; Transplantation, Heterologous
• ISSN: 0008-543X; 1097-0142
• DOI: 10.1002/1097-0142(19940201)73:3+<800::AID-CNCR2820731309>3.0.CO;2-P

Background. The prognosis of pancreatic adenocarcinoma still remains poor because of the lack of reliable diagnostic tests for early stages of the disease. Monoclonal antibody 17‐1A(MoAb 17‐1A) has been studied extensively, and the antigen recognized by MoAb 17‐1A is expressed by adenocarcinomas of the pancreas and stomach, as well as other normal and malignant epithelial tissues. The potential of MoAb 17‐1A was investigated for its ability to detect pancreatic carcinomas. The use of MoAb 17‐1A in treatment also was studied. Methods. Immunoreactivity of MoAb 17‐1A with human pancreatic carcinoma cell line HuP‐T4 was examined histochemically by the avidin‐biotinylated enzyme complex method. MoAb 17‐1A was labeled with 125I by the Iodogen method and 111In using either diethylenetriaminepentaacetic anhydride (cDTPA) or 1‐(p‐benzyldiazonium) diethylenetriaminepentaacetic acid (aDTPA). After injection in nude mice bearing HuP‐T4 xenografts, the biodistribution of 111In‐ and 125I‐labeled MoAb 17‐1A was examined at various time points. Results. Positive staining of MoAb 17‐1A was noted for HuP‐T4 cells. A statistically significant (P < 0.01) greater tumor uptake was observed at 3 days after intravenous injection of 125I‐labeled MoAb 17‐1A when compared with 125I‐labeled nonspecific immunoglobulin G. 125I‐ and 111In‐labeled MoAb 17‐1A was concentrated in HuP‐T4 carcinoma 1.9–4.8 times higher than in the spleen, heart, liver, and pancreas. Conclusions. MoAb 17‐1A was found to bind selectively to human pancreatic carcinoma HuP‐T4. Tumor exhibited higher uptake of radiolabeled MoAb 17‐1A compared with adjacent normal tissues. These results suggest that MoAb 17‐1A may be applicable to the radioimmunodetection and radioimmunotherapy of pancreatic adenocarcinomas. Cancer 1994; 73:800–7.