Analysis of a region from the bacteriophage resistance plasmid pCI528 involved in its conjugative mobilization between Lactococcus strains

A 10-kb HindIII fragment of pCI528 cloned into the nonconjugative shuttle vector pCI3340 could be transferred by conjugative mobilization from Lactococcus lactis subsp. lactis MG1363, whereas other HindIII fragments of pCI528 or the vector alone were nonmobilizable. Subcloning of this 10-kb region i...

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Bibliographic Details
Published in:Journal of Bacteriology 1993-09, Vol.175 (18), p.6002-6009
Main Authors: Lucey, M, Daly, C, Fitzgerald, G
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Bacterial Proteins - genetics
BACTERIOFAGOS
Bacteriology
BACTERIOPHAGE
Bacteriophages - physiology
Base Sequence
Biological and medical sciences
Biotechnology
Conjugation, Genetic - genetics
DNA, Bacterial
Fundamental and applied biological sciences. Psychology
Genes
Genetics
INFECCION
INFECTION
LACTOCOCCUS LACTIS
Lactococcus lactis - genetics
Microbiology
Molecular Sequence Data
Open Reading Frames
PLASMIDE
PLASMIDIOS
Plasmids - genetics
Repetitive Sequences, Nucleic Acid
Restriction Mapping
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
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Description
Summary:A 10-kb HindIII fragment of pCI528 cloned into the nonconjugative shuttle vector pCI3340 could be transferred by conjugative mobilization from Lactococcus lactis subsp. lactis MG1363, whereas other HindIII fragments of pCI528 or the vector alone were nonmobilizable. Subcloning of this 10-kb region identified a 4.4-kb BglII-EcoRI fragment which contained all the DNA essential for transfer. Sequence analysis of a 2-kb region within this 4.4 kb-segment revealed a region rich in inverted repeats and two potential overlapping open reading frames, one of which demonstrated homology to mobilization proteins of two nonconjugative staphylococcal plasmids
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.18.6002-6009.1993