Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants

• Published in: Proceedings of the National Academy of Sciences - PNAS 1993-06, Vol.90 (12), p.5474-5478
• Main Authors: Perlman, Riki, Yablonski, Deborah, Simchen, Giora, Levitzki, Alexander
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Carrier Proteins; Cell Cycle Proteins; Cell growth; Classical genetics, quantitative genetics, hybrids; clonacion; clonage; cloning; Cloning, Molecular; code genetique; codigo genetico; Diploidy; expresion genica; expression des genes; Fundamental and applied biological sciences. Psychology; Fungal Proteins - genetics; Gene Deletion; Gene Expression; gene products; Genes; Genes, Fungal; Genes, Mating Type, Fungal; Genes, Suppressor; genetic code; Genetic screening; genetica; Genetics; Genetics of eukaryotes. Biological and molecular evolution; genetique; Genomics; genotipos; Genotype; genotypes; levadura; levure; mating; Mating Factor; Molecular Sequence Data; mutant; mutantes; mutants; Mutation; nucleotide sequence; Peptides - genetics; Peptides - physiology; Pheromones; Pheromones - genetics; Pheromones - physiology; plasmide; plasmidios; Plasmids; predictions; Proteins; ras-GRF1; Reproduction; Restriction Mapping; RNA; RNA, Messenger - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - physiology; Saccharomyces cerevisiae Proteins; secuencia nucleica; Sequence Homology, Amino Acid; sequence nucleique; STE5 gene; Temperature; temperature-sensitive mutant; Thallophyta, bryophyta; Transcription, Genetic; Vegetals; Yeast; Yeasts
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.90.12.5474

The STE5 gene of Saccharomyces cerevisiae was cloned using a screening procedure designed to isolate genes of the S. cerevisiae pheromone response pathway. We screened a yeast genomic high-copy-number plasmid library for genes that allow mating of cdc25ts mutants at the restrictive temperature without affecting the cell-cycle-arrest phenotype. One of the genes cloned was identified by genetic analysis as STE5. STE5 encodes a predicted open reading frame of 916 amino acids and exhibits significant homology to Far1 protein. RNA blot analysis reveals that STE5 gene transcription is regulated by the mating type of the cell and depends on an intact pheromone-response pathway.