Determination of cyclophosphamide metabolites by gas chromatography and thermionic specific detection interindividual differences in hepatic biotransformation of cyclophosphamide in man in vitro

Sensitive methods for the determination of the cyclophosphamide metabolites nornitrogen mustard, 4-ketocyclophosphamide and carboxyphosphamide are presented. After liquid-liquid extraction and derivatization, the metabolites are determined by gas chromatography and thermionic specific detection. The...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1995-11, Vol.673 (2), p.205-212
Main Authors: Sessink, P.J.M., Vaes, W.H.J., van den Broek, P.H.H., Noordhoek, J., Bos, R.P.
Format: Article
Language:English
Subjects:
Chromatography, Gas - methods
Chromatography, Gas - statistics & numerical data
Cyclophosphamide - analysis
Cyclophosphamide - metabolism
Cyclophosphamide - pharmacokinetics
Humans
Liver - chemistry
Liver - metabolism
Nitrogen - analysis
Phosphoramide Mustards - analysis
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Summary:Sensitive methods for the determination of the cyclophosphamide metabolites nornitrogen mustard, 4-ketocyclophosphamide and carboxyphosphamide are presented. After liquid-liquid extraction and derivatization, the metabolites are determined by gas chromatography and thermionic specific detection. The methods were used to study the in vitro biotransformation of cyclophosphamide with S-9 liver fractions of human donors. The results show large interindividual differences in the formation of nornitrogen mustard and carboxyphosphamide. 4-Ketocyclophosphamide was not detected.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(95)00264-7