Isolation and molecular analysis of the gene for cytochrome c1 from Kluyveromyces lactis

By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of th...

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Bibliographic Details
Published in:Current genetics 1996-07, Vol.30 (2), p.145
Main Authors: Gbelská, Y, Horváthová, K, van der Aart, Q J, Zonneveld, B J, Steensma, H Y, Subík, J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Chromosome Mapping
Chromosomes, Fungal
Cloning, Molecular
Cytochromes c1 - genetics
Cytochromes c1 - metabolism
Genes, Fungal
Kluyveromyces - enzymology
Kluyveromyces - genetics
Kluyveromyces - growth & development
Mitochondria - genetics
Molecular Sequence Data
Mutation
Sequence Homology, Amino Acid
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Summary:By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of these mutants we have cloned the structural gene for cytochrome c1, named KlCYT1. This gene has been assigned to chromosome VI and its nucleotide sequence exhibited 74.3% identity to the homologous gene of S. cerevisiae.
ISSN:0172-8083
DOI:10.1007/s002940050113