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Cloning and expression of full-length Trichoderma reesei cellobiohydrolase I cDNAs in Escherichia coli

The process of converting lignocellulosic biomass to ethanol via fermentation depends on developing economic sources of cellulases. Trichoderma reesei cellobiohydrolase (CBH) I is a key enzyme in the fungal cellulase system; however, specific process application requirements make modification of the...

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Published in:	Applied biochemistry and biotechnology 1996, Vol.57, p.389-398
Main Authors:	Laymon, R.A. (National Renewable Energy Laboratory, Golden, CO.), Adney, W.S, Mohagheghi, A, Himmel, M.E, Thomas, S.R
Format:	Article
Language:	English
Subjects:	Base Sequence Biological and medical sciences Biotechnology Cellulase - biosynthesis Cellulase - genetics Cellulose 1,4-beta-Cellobiosidase CLONACION CLONAGE Cloning, Molecular DNA, Complementary - biosynthesis ESCHERICHIA COLI Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Genetic engineering Genetic technics Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Recombinant Proteins - biosynthesis RNA, Messenger - isolation & purification Thioredoxins - biosynthesis Thioredoxins - genetics TRICHODERMA
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container_title	Applied biochemistry and biotechnology
container_volume	57
creator	Laymon, R.A. (National Renewable Energy Laboratory, Golden, CO.) Adney, W.S Mohagheghi, A Himmel, M.E Thomas, S.R
description	The process of converting lignocellulosic biomass to ethanol via fermentation depends on developing economic sources of cellulases. Trichoderma reesei cellobiohydrolase (CBH) I is a key enzyme in the fungal cellulase system; however, specific process application requirements make modification of the enzyme by site-directed mutagenesis (SDM) an attractive goal. To undertake SDM investigations, an efficient, cellulase-free host is required. To test the potential of Escherichia coli as a host, T. reesei CBH I cDNA was expressed in E. coli strain GI 724 as a C-terminal fusion to thermostable thioredoxin protein. Full-length expression of CBH I was subsequently verified by molecular weight, Western blot analysis, and activity on soluble substrates
doi_str_mv	10.1007/BF02941718
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source	Springer Online Journal Archives (Through 1996)
subjects	Base Sequence Biological and medical sciences Biotechnology Cellulase - biosynthesis Cellulase - genetics Cellulose 1,4-beta-Cellobiosidase CLONACION CLONAGE Cloning, Molecular DNA, Complementary - biosynthesis ESCHERICHIA COLI Fundamental and applied biological sciences. Psychology Gene Expression Gene Library Genetic engineering Genetic technics Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Recombinant Proteins - biosynthesis RNA, Messenger - isolation & purification Thioredoxins - biosynthesis Thioredoxins - genetics TRICHODERMA
title	Cloning and expression of full-length Trichoderma reesei cellobiohydrolase I cDNAs in Escherichia coli
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