Serodiagnosis of Chagas disease: new reagent for the indirect hemagglutination test (THAI(IAL))

A new reagent was designed to the indirect hemagglutination test (IHATIAL), utilizing goose red blood cells as inert matrix and standardized for the field diagnosis of American trypanosomiasis. The objective was to substitute the lyophilized or frozen reagent of IHAT produced routinely using human e...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 1996-03, Vol.29 (2), p.137
Main Authors: Sasaki, A T, Hoshino-Shimizu, S, Nakamura, P M, Vaz, A J, Camargo, E D, da Silva, M V
Format: Article
Language:Portuguese
Subjects:
Animals
Chagas Disease - diagnosis
Geese - blood
Geese - immunology
Hemagglutination Tests - methods
Humans
Indicators and Reagents
Sensitivity and Specificity
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Summary:A new reagent was designed to the indirect hemagglutination test (IHATIAL), utilizing goose red blood cells as inert matrix and standardized for the field diagnosis of American trypanosomiasis. The objective was to substitute the lyophilized or frozen reagent of IHAT produced routinely using human erythrocytes in the Adolfo Lutz Institute (São Paulo/Brazil). The standardized reagent presented a long stability in liquid suspension, and was evaluated in 137 serum samples from patient with and without Chagas' disease, by IHATIAL. The diagnostic performance of this test was similar to the IHAT utilizing human erythrocytes and to that of a commercial IHAT kit. The sensitivity was 1.00, specificity 0.98, predictive value of positive 0.96 and of negative 1.00. Different batches of reagent successively produced proved to be reproducible in a quality control method. The new reagent is more economic than the former reagent, it can be produced easily and may be applicable to the seroepidemiologic studies.
ISSN:0037-8682